Many human disorders are age-related whereby their prevalence and severity increase in aging patients. For example, level of coagulation factor, human factor IX, increases in aging subjects and is potentially involved in cardiovascular disorders. A better understanding of the molecular mechanisms of age-associated diseases is needed.
Researchers at the University of Michigan have identified age-regulatory sequences and developed a recombinant expression vector that incorporates these sequences in the expression for human factor IX protein. Host cells and non-human transgenic animals containing this expression vector were used to investigate age-dependent expression of human factor IX. These vectors can also be used to determine the age-related regulatory activity of candidate nucleic acid sequences.
Applications and Advantages
- Expression vector for understanding age-regulation effect on gene expression.
- Regulation of factor IX expression in blood clotting disorders such as hemophilia.
- Understanding of association between human factor IX expression and age-related cardiovascular and thrombotic diseases.
- Transgenic animals can be used as animal models for age-related diseases.
- Can be applied for regulation of a variety age-related and liver-related disorders gene expression.
Publications:“Genetic mechanisms of age regulation of human blood coagulation factor IX” Science (1999), 285, p. 739.
A patent has been issued for this invention (US 6,610,906)