Endotoxic shock is a condition, provoked by the release of lipopolysaccharide (LPS) from the outer membrane of most gram negative bacteria (e.g., Escherichia coli; Salmonella tymphimurium). One example of a condition involving Endotoxic shock is sepsis. Sepsis is a systemic reaction characterized by arterial hypotension, metabolic acidosis, decreased systemic vascular resistance, tachypnea and organ dysfunction. Sepsis can result from septicemia. Sepsis is a major cause of morbidity and mortality in humans and other animals. Despite the major advances of the past several decades in the treatment of serious infections, the incidence and mortality due to sepsis continues to rise.
Researchers at the University of Michigan have developed methods for using PON3 genes and peptides in the treatment of endotoxemia, oxidative damage, chemical toxicity, and other conditions. The present invention provides isolated nucleotide sequence encoding PON3, wild type and mutant PON3 peptides. The invention also provides an assay for the screening of drugs that affect lactone metabolism or other biological activities of PON3.
Applications and Advantages
- Therapeutic treatments for sepsis, oxidative damage, and chemical toxicity
- Easy to produce
- Low cost