Office of Technology Transfer – University of Michigan

Inline Electrochemical Detection for Capillary Electrophoresis

Technology #2120

Background

Capillary electrophoresis (CE) and other analytical techniques have greatly benefited from micromachining. Microfabrication technology provides a means for implementation of complex CE devices with a high degree of functionality, yielding fast and highly efficient separations. The majority of micromachined CE devices, however, have been fabricated using bonded, glass substrate technology with off-chip laser induced fluorescence detection. The advantages of miniaturization are greatly reduced when large detection systems are used in microanalysis.

Technology

Researchers at the University of Michigan have developed microscale electrophoresis devices using microfabrication for separation and detection of biomolecles and chemicals. This microscale CE device is comprised of an electrochemical inline detection system with electrophoresis channels on a substrate platform, with reservoirs, sieving matrix, and inline detection system.

Applications and Advantages

Applications

  • separation and detection of biomolecules and-nl-chemicals

Advantages

  • use of inline electrochemical methods in-nl-capillary electrophoresis
  • inexpensive, easy fabrication
  • improved resolution and a higher limits-nl-of detection