Fluorescence measurements are an invaluable tool for a wide variety of applications in various fields, including chemistry, biomedical science, and clinical research. A primary advantage of measuring fluorescence over absorption is its high selectivity and sensitivity. One of the most important considerations for a fluorescence detection system is to separate fluorescence signals from excitation light. Most of the systems to date can only excite and detect a limited number of fluorophores with a single excitation wavelength. If broadband light sources such as xenon lamps are sometimes used, excitation filters are often used. As The types of fluorophores that can be simultaneously excited are limited, and as such, conventional fluorescence measurements have not only reduced the detection speed and sensitivity, but have also limited the selection of detectable fluorescent markers.
Researchers at the University of Michigan have developed a fluorescence detection system for testing samples that allows for a single laser source to simultaneously excite a wide variety of fluorophores in a wide wavelength range, from visible to near infrared. This technology also differs from existing conventional fluorescence collection devices as band pass filters and dichroic mirrors are not necessary components, thus significantly simplifying the optical configurations. In particular, the detection system comprises a white light generation system which outputs a white light pulse which excites the fluorophores. A time-resolving detector receives the fluorescence and at least a portion of the white light pulse and separates the fluorescence from the portion of the white light pulse.
Applications and Advantages
- Fluorescence detection for various applications-nl-including flow cytometry and fluorescence-nl-screening and microscopy
- Detection of whole spectrum