Chromatography separates mixtures into individual components by passing them through columns containing specialized media. The separated components elute from the column individually over time, and parameters such as absorbance or fluorescence is used to record their presence. In some experiments, it is desirable to collect these separated components for subsequent workup. In these instances, it is necessary to know which well the desired samples/ particular peaks are located.
This software, currently designed for the Beckman-Coulter FC-204 fraction collector as used with their PF2D Proteome System, presents a graphical plot of a user selected chromatography data set as intensity vs time. It overlays indications of where in time each fraction was collected. Also displayed at each fraction is its plate number and well position. Additionally, a movable cursor can be placed onto a peak of interest and a visual indication of that peak’s plate number and well position given. An expanded intensity-time plot of that fraction is provided in a separate window for detailed analysis. Parameters including chromatography run number, chromatography detector to fraction collector lag time, fraction collector number of wells per plate, fraction collector start and end times and fraction duration are accounted for. Hard copy printed output of the chromatography data set with each fraction’s time, plate number and well position is provided for. This software makes identification of well position easy for any peak in the separation process.
Applications and Advantages
- Determining the well position of any peak-nl-in a chromatography experiment
- Detailed information in an easy to use-nl-application
- Easily identify the position and make up of-nl-specific runs in an experiment