In various biomedical applications including diagnostics, tissue engineering, and basic study of cell adhesion and motility, there is a growing need for controlled and stable immobilization of one or more (bio)molecules onto a substrate. While several approaches exist to immobilize a single type of molecule to a substrate, techniques to simultaneously present controlled ratios of multiple molecules are not as readily available.
This invention is based on chemical vapor deposition (CVD) polymerization technique to multiple substituted [2.2]paracyclophanes on a substrate. Using this coating technology, surfaces can present a variety of functional groups including amines, esters, aldehydes, and alcohols, which can subsequently be utilized to covalently bind (bio)molecules. As the ratios of starting monomers is controllable, so too is the ratio of functional groups present on the modified surface. The CVD process is a one-step coating technique, and is performed without any solvents or catalysts, which minimizes surface contamination. Moreover, this technique can be applied to a variety of 3-dimensional structures and the coatings are stable and robust in various environments.
Applications and Advantages
- Biomedical diagnostics/assays
- Micrototal analytical systems
- Tissue engineering
- Cell shape/adhesion/motility studies
- Flexibility functionalizing material surface-nl-without altering bulk properties
- Modular format
- One-step process without any need for post-treatment