Spatially-controlled immobilization of molecules, biological ligands, proteins or cells on substrates is important for various technologies including biological assays, combinatorial screening of drugs, and biosensors. For example in tissue engineering, specific 3-dimensional architecture that directs cells to occupy defined locations on an implant or device, is necessary in the appropriate formation of tissue or organized cell structures. For biologically-active interfaces, strategies for the specific and robust attachment of biological ligands onto the surfaces are essential. As such, stable immobilization of one or multiple types of biomolecules to a surface has been identified as a critical challenge.
Researchers at the University of Michigan have developed a method using chemical vapor deposition (CVD) of substituted xylylenes to provide robust functionalized polymer composition on a substrate. The CVD technique with such polymer systems is a one-step coating process that retains bulk properties of a material, while enhancing surface contact properties. Functional group introduced to the surface via CVD and that of the ligand can then further undergo “click” chemistry for modular regioselective reactions.
Applications and Advantages
- Surface biofunctionalization: biomedical-nl-diagnostics/assays, tissue engineering
- Cell shape/adhesion/motility studies
- Simple coating procedure
- Process applicable to various surfaces
- Chemistry that is stable, with minimal-nl-cross-reactivity, high conversions/yields,-nl-minimal side products, and proceed under-nl-relatively benign reaction conditions