Office of Technology Transfer – University of Michigan

Nested Landing Pad System for Synthetic Biology

Technology #3549

Synthetic biology techniques aim to provide insight into complex systems by development and study of genetic circuits based on incorporation of genetic information into bacteria. Current techniques for gene- incorporation and editing are clumsy, inadequate and expensive and often result in mismatches and errors. Large amounts of genetic information are also difficult to work with in current systems. More efficient and simpler systems for synthetic biology are required to facilitate advances in biopharmaceuticals, genetic engineering and biotechnology.

Recursive construction of genetic circuits using landing pads

There is much to be learned from the construction and study of synthetic genetic circuits with technologies that allow for simple incorporation of genetic elements into biological systems. Using a series of “landing pads” a new system uses recursive incorporation of genetic material in order to accommodate larger genetic programs and decrease the amount of troubleshooting required. Additional DNA barcode technology lowers costs to the user by helping to characterize the DNA insertion by PCR so that the user does not need to sequence at each step. Recursive incorporation of genetic elements for incremental building and testing is a simple and effective way to create and learn from genetic circuits.

Applications

  • Creation of synthetic genetic circuits
  • Testing of genetic circuits
  • Expression of multiple genes
  • Engineering & health research
  • Educational settings

Advantages

  • Genetic bar coding for screening
  • Large genetic programs accessible
  • Easy to troubleshoot
  • Not necessary to sequence at each step
  • Regulated protein expression
  • Saves time and money