Office of Technology Transfer – University of Michigan

SiMREPS Analysis Software - v 1.0

Technology #6250-1

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SiMREPS Analysis Software, Version 1.0 - Academic Research Use License
Nils G. Walter
External Link (
Managed By
Drew Bennett
Associate Director - Software Licensing 734-615-4004
Patent Protection

US Patent Pending

This package contains installers for two programs that perform single-molecule kinetic fingerprinting (SiMREPS=single-molecule recognition through equilibrium Poisson sampling) analysis using one or more grayscale TIF stacks (movies) as input. The first program generates intensity-versus-time traces form grayscale TIF stacks for subsequent kinetic analysis. The second program performs kinetic analysis of the intensity-versus-time traces to determine whether each trace corresponds to nonspecific binding or specific binding to the target analyte, using a Parameters file to establish thresholds for this classification of traces. This method provides:

·  Extremely low-background detection of single biomolecules (when probe design and analysis parameters are sufficiently optimized)

·  Quantification with a dynamic range of typically ~2-3 orders of magnitude

·  Ultra-high specificity capable of detecting single-base mutations in a 1 million-fold excess of wild-type sequence

See User Guide for more information, including links to example input and output files for testing.