Office of Technology Transfer – University of Michigan

The cryopreservation of human tissue samples for subsequent culture of live tissue

Technology #7532

Over the past several decades, there has been an increasing need for better cryopreservation of tissue samples as well as increased research to tackle this problem. To-date there is a large amount of media used for the cryopreservation of cells from culture. However, there are still issue with freezing and thawing live tissue samples. This is largely due to crystallization that occurs on the tissue making it unable to be used in subsequent culturing techniques. The ability to freeze, thaw, and re-grow live tissue samples would prove useful in scientific research, medical practices, and storage in tissue banks and bio-banks.

Novel method for the cryopreservation of live tissue samples followed by subsequent culturing

This novel technology encompasses a technique that allows live tissue samples to efficiently frozen, stored, and then thawed and used for culturing purposes. Not only can this technique work on cells initially taken from a cell culture, but it can be used on live tissue such as from biopsies. Using this method, live epithelia tissue samples from a colon biopsy were cryopreserved for 10 days and then allowed to grow in culture, which formed distinct and correct cell growth after three days. This technique has the ability to transform the way that we store live tissue samples to increase their use and longevity for research purposes as well as the ability to ship samples for examination and use world-wide.

Applications

  • Cryopreservation of live tissue samples
  • Shipment of live tissue samples
  • Bio-banking of live tissue samples

Advantages

  • Use of live tissue samples